Journal: Cell Reports Medicine
Article Title: RSAD2 : A pathogenic interferon-stimulated gene at the maternal-fetal interface of patients with systemic lupus erythematosus
doi: 10.1016/j.xcrm.2025.101974
Figure Lengend Snippet: RSAD2 is highly expressed in pregnant patients with SLE (A) Heatmap showing the expression of interferon-stimulated genes (ISGs) in the peripheral blood mononuclear cells (PBMCs) of healthy women (Healthy PBMCs), female patients with SLE (SLE PBMCs), healthy pregnant women (Healthy pregnancy PBMCs), and pregnant patients with SLE (SLE pregnancy PBMCs). (B) Heatmap showing the expression of ISGs in the decidua and villi of healthy early pregnant women and pregnant patients with SLE. (C) Heatmap showing the expression of ISGs in the decidual and trophoblast organoids from the first trimester decidual gland or trophoblast cells (passage 1, day 5), treated with 1,000 U/mL IFN-α or PBS for 12 h. (A, B, and C). The 2 −ΔCt values of ISGs were calculated from the ISG qPCR array data. (D) Representative IHC image of IFNAR1 and CD55 (expressed in DSCs) co-staining of decidual tissue sections (top), and representative IHC image of IFNAR1 and EPCAM (expressed in VCTs, the proliferative villous cytotrophoblast cells) co-staining of chorionic villi sections (bottom); scale bar, 50 μm. (E) Representative IHC image of decidual organoids (top) from the first trimester decidual glands (passage 1, day 5) and of trophoblast organoids (bottom) from the first trimester trophoblast cells (passage 1, day 5); scale bar, 50 μm. (F) Expression of ISGs in decidual CD56 + NK cells, CD3 + T cells, CD14 + macrophages, decidual stromal cells (DSCs), and trophoblasts ( n = 3); the 2ˆ −ΔΔCt values of ISGs were calculated from the qPCR data. (G and H) Western blot showing RSAD2 and ISG15 expression in DSCs and trophoblasts, treated with 1,000 U/mL IFN-α or PBS for 12 h. Data were analyzed using the unpaired t test and are presented as the mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns , not significant.
Article Snippet: Human physical examination peripheral blood samples , First Affiliated Hospital of Anhui Medical University , N/A.
Techniques: Expressing, Staining, Western Blot
